LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add 100–150 µL of the combined LIVE/DEAD® assay reagent and incubate the cells for 30–45 minutes at room temperature	Following the dye incubation, add about 10 µL of the fresh LIVE/DEAD® reagent solution or D-PBS to a clean microscope slide.

Protocol tips
Add 100–150 µL of the combined LIVE/DEAD® assay reagent and incubate the cells for 30–45 minutes at room temperature
Downstream tips
Following the dye incubation, add about 10 µL of the fresh LIVE/DEAD® reagent solution or D-PBS to a clean microscope slide.

Publication protocol

Immunohistochemistry and live-dead assays were performed in 96 well plates using a Hermes Wiscan instrument (IDEA Bio-medical, Rehovot, Israel). Cells were plated and treated with drugs for 12–24 h as indicated in the figure legend. After treatment plates were centrifuged to deposit floating cells onto the plate (800 rpm, 5 min). Cells were then either fixed in place (4% paraformaldehyde in PBS) or were subjected to live-dead assay using a commercially available kit and performed according to the manufacturer's method (Life Technologies, Grand Island, NY). Cell viability was measured using Wisoft software. Fixed cells were subjected to Ki67 / IL-6 / TNFα staining using standard procedures.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells below.

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