CellTiter-Glo® Luminescent Cell Viability Assay

Live / Dead assay mammalian cells - GH3

Experiment
Live / Dead assay mammalian cells - GH3
Product
CellTiter-Glo® Luminescent Cell Viability Assay from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
Add reagent and mix contents for 2 minutes to induce cell lysis.

Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Publication protocol

Cell viability was measured using the MTS-based Cell Titer 96®AQueous One solution cell proliferation assay (Promega, Madison, WI) or CellTiter-Glo® luminescent cell viability assay (Promega) according to the manufacturer's instructions. Upon addition of MTS solution, the reaction plate was incubated at 37°C for 3 h, and the absorbance was read at 490 nm with a plate reader (TECAN, Männedorf, Switzerland). For phosphatidylserine exposure, cells were stained with annexin V-PE as described by the manufacturer (BD Biosciences, San Jose, CA), and assayed by flow cytometry (CyAn ADP, Beckman Coulter, Brea, CA, USA)

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Papers

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Manufacturer protocol

Download the product protocol from Promega for CellTiter-Glo® Luminescent Cell Viability Assay below.

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