Protocol tips |
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Add 3 µL of the dye mixture for each mL of the bacterial suspension. Mix thoroughly and incubate at room temperature in the dark for 15 minutes |
LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific has not yet been reviewed for this experiment
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Discussion
4 years ago
Author: M. Daecher
Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?
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