LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy

Live / Dead assay bacteria - Mycobacterium tuberculosis

Experiment
Live / Dead assay bacteria - Mycobacterium tuberculosis
Product
LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Mix 35 µL of Component A with 35 µL of Component B in a microfuge tube.

Add 6 µL of the combined reagent mixture to te tubes.

Incubate at room temperature in the dark for 15 minutes

Publication protocol

Infected M1-MDMs with knockdown of Rev-erbα or ectopically overexpressed Rev-erbα were lysed with 0.06% SDS after 24 h of incubation, and bacterial suspensions were used with the LIVE/DEAD BacLight bacterial viability and counting kit as per the manufacturer's instructions (Invitrogen). The percentage of live and dead bacteria was determined by flow cytometry (BD FACSCalibur, BD Biosciences) after staining with SYTO9 and propidium iodide. For CFU determination, after macrophage solubilization, the bacterial suspensions were serially diluted, 50 μl of each sample was plated, and CFU were counted. Final calculations included the dilution factor and the volume of diluted sample for plating. Anti-IL10 neutralizing antibody (purified Rat anti-human IL10, BD Biosciences) or isotype control was used 30 min prior to infection with H37Ra or H37Rv.

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Discussion

Discussion

2 years ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Mycobacterium tuberculosis using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific.

Paper title
Human Gene Repression by Rev-erbα Ameliorates Clearance
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy below.

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