LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy

Live / Dead assay bacteria - Porphyromonas gingivalis

Experiment
Live / Dead assay bacteria - Porphyromonas gingivalis
Product
LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Add 3 µL of the dye mixture for each mL of the bacterial
suspension.

Mix thoroughly and incubate at room temperature in the dark for 15 minutes.

Publication protocol

The biofilm from the MRD was stained using a Live/Dead® BacLight™ Bacterial Viability Kit (L7007; Molecular Probes, Eugene, OR) and observed by confocal laser scanning microscopy (LSM-700; Carl Zeiss, München-Hallbergmoos, Germany) as described previously55.

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Discussion

Discussion

4 years ago

Author: M. Daecher Germany

Live/dead assay Bacteria

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Live / Dead assay bacteria - Porphyromonas gingivalis using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy from Thermo Fisher Scientific.

Paper title
Aggravation of collagen-induced arthritis by orally administered through modulation of the gut microbiota and gut immune system
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy below.

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