Live/Dead Double Staining Kit (Merck)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Prior to use, mix 1 µl of solution A and 1 µl of solution B in 1ml of Staining Buffe	Resuspend to 1 ml Staining Solution. Incubate for 15 minutes at 37°C

Upstream tips
Prior to use, mix 1 µl of solution A and 1 µl of solution B in 1ml of Staining Buffe
Protocol tips
Resuspend to 1 ml Staining Solution. Incubate for 15 minutes at 37°C

Publication protocol

Cell viability after exposure to colistin sulfate was assessed using a LIVE/DEAD staining kit (EMD Chemicals Millipore 50-231-0606). Stains were prepared per manufacturer’s operating procedure. After staining, 10 µL of sample was injected into an INCYTO C-chip disposable hemocytometer (DHC-N01). Cells were imaged using a fluorescence microscope, and the number of PA14 cells that were alive after exposure was determined using IMAGE J (ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/).

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Manufacturer protocol

Download the product protocol from Merck Millipore for Live/Dead Double Staining Kit (Merck) below.

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