|Sigma CRISPR plasmid products are delivered as mini-prep aliquots,
which may not be suitable for transfection into particular cell types. For best results, use maxi-prepping plasmids using endotoxin-free DNA purification kits prior to transfection
CMV-CAS9-2A-GFP Plasmid from Sigma-Aldrich has not yet been reviewed for this experiment
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1 year ago
Author: Milena Alexeyeva
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
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