EZ DNA Methylation kit

DNA methylation profiling Gene specific profiling - Caco-1 CHST11

Experiment
DNA methylation profiling Gene specific profiling - Caco-1 CHST11
Product
EZ DNA Methylation kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.

Publication protocol

Bisulfite Treatment and DNA Purification
Genomic DNA was chemically modified using sodium bisulfite, which converts all unmethylated cytosine to uracil but leaves methylated cytosine unmodified. To this end, we used an EZ DNA methylation kit (Zymo Research, Irvine, CA) according to the manufacturer's instructions. In brief, genomic DNA was treated with sodium bisulfite for 2.5 hours at 65°C and desulfonation was performed for 20 minutes at room temperature. Bisulfite-converted DNA was purified using a Zymo-Spin IC column (Zymo Research) and eluted with 10 μL of distilled water. The eluted DNA was either used immediately for methylation analysis or was stored at −20°C until further use.

Full paper   Login or join for free to view the full paper.

Reviews

EZ DNA Methylation kit from Zymo Research has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

2 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - Caco-1 CHST11 using EZ DNA Methylation kit from Zymo Research.

Paper title
Genome-wide identification and validation of a novel methylation biomarker, SDC2, for blood-based detection of colorectal cancer.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Gene specific profiling - Caco-1 CHST11 using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms