Infinium MethylationEPIC Kit

DNA methylation profiling Gene specific profiling - MCF-7 LINE1

Experiment
DNA methylation profiling Gene specific profiling - MCF-7 LINE1
Product
Infinium MethylationEPIC Kit from Illumina
Manufacturer
Illumina

Protocol tips

Upstream tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

2.4. Genome-wide methylation assay
The genome-wide methylation assay was carried out as described previously [23]. Briefly, 2 μg of chromosomal DNA isolated from a 60-mm culture dish was applied to an Illumina Infinium Human Methylation EPIC Beadchip (Illumina, San Diego, CA, USA) to detect genome-wide methylation. A methylation index (β) was calculated for each site, which is a variable ranging from 0 (no methylation) to 1 (100% methylation). The β value was then used to calculate a ratio of relative methylation between the control and Rh2-treated cells. The array data is accessible from the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/) with the accession number GSE93356.

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Reviews

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Discussion

Discussion

2 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

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Manufacturer protocol

Download the product protocol from Illumina for Infinium MethylationEPIC Kit below.

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Videos

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