CpGenome Universal DNA Modification Kit

DNA methylation profiling Gene specific profiling - A2780 TGFBI

Experiment
DNA methylation profiling Gene specific profiling - A2780 TGFBI
Product
CpGenome Universal DNA Modification Kit from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP)
DNA from tissue samples and cell lines were subjected to bisulfite treatment using CpGgenome DNA Modification Kit (Chemicon, USA). Sequences, Tm, and product length of each primer used for MSP and BSP analysis are summarized in Table The band expanded with methylation-specific PCR primers corresponding to the DNA methylation in the promoter region was marked as "M". The band expanded with non-methylation-specific primers was marked as "U".



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Reviews

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Discussion

Discussion

2 years ago

Author: Jody Hancock Canada

How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Gene specific profiling - A2780 TGFBI using CpGenome Universal DNA Modification Kit from Millipore.

Paper title
TGFBI promoter hypermethylation correlating with paclitaxel chemoresistance in ovarian cancer
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Manufacturer protocol

Download the product protocol from Millipore for CpGenome Universal DNA Modification Kit below.

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Videos

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