EpiTect MSP Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.

Publication protocol

MSP
MSP was carried out in a total volume of 50 μl (EpiTect Master Mix, 25 μl), with modified DNA (4 μl) in RNase-free water. The reaction conditions for MSP were: initial denaturation at 95°C for 10 min, followed by 94°C for 30 sec; 58°C for 45 sec; 72°C for 45 sec for 40 cycles; and 72°C for 10 min for both methylated and unmethylated reactions. The PCR products (6 μl) were electrophoresed, verified in 1.5% agarose gel and observed under UV light. Target gene amplification of methylation primers was considered fully methylated, while that of unmethylated primers was considered partially methylated.

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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect MSP Kit below.

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