DreamTaq DNA Polymerases

PCR Conventional / Qualitative PCR - bacterial DNA

Experiment
PCR Conventional / Qualitative PCR - bacterial DNA
Product
DreamTaq DNA Polymerases from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp

Publication protocol

Conventional and real-time PCR amplifications
Amplification reactions with the two selected primer pairs, one specific for the strains of 1 and the second specific for 2, were performed in a Biometra T3000 thermocycler (Biometra, Göttingen, Germany). The reaction mixture in 15 μl of total reaction volume contained 10 ng of DNA, 0.4 U of Dream DNA Polymerase (Promega, Madison, WI, USA), 1× reaction Dream Taq Green buffer (Thermo Scientific, Vilnius, Lithuania), 0.15 mM dNTPs and 0.7 mM of each primer. The following experimentally determined amplification conditions were used: initial denaturation at 94 °C for 4 min; 30 cycles at 94 °C for 45 s, 55–62 °C for 45 s for primers Psm1-6F and Psm1-6R (for detection of 1 strains) and 50–58 °C for 45 s for primers Psm2-8F and Psm2-8R (for detection of 2 strains) and 72 °C for 1 min; and final extension at 72 °C for 10 min. The resulting PCR products were separated by electrophoresis on 1.5 % agarose gels as described above.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing PCR Conventional / Qualitative PCR - bacterial DNA using DreamTaq DNA Polymerases from Thermo Fisher Scientific.

Paper title
Development of SCAR markers for rapid and specific detection of pv. races 1 and 2, using conventional and real-time PCR
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DreamTaq DNA Polymerases below.

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