|Amplicon size should be consistent for each target sequence and limited to approximately 65 - 100 bp.|
|PCR plates, tubes and tips should be UV sterilized for 20-30 mins|
QuantiFast Multiplex PCR Kit from Qiagen has not yet been reviewed for this experiment
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3 years ago
Author: R. Verma
I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?
3 years ago
Author: Roland Lafontaine
Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.
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