LC3B Antibody

Autophagy assay cell type - RAW 264.7

Experiment
Autophagy assay cell type - RAW 264.7
Product
LC3B Antibody from Novus Biologicals
Manufacturer
Novus Biologicals

Protocol tips

Protocol tips
Cells were cultured in normal culture medium under normoxia (21% O2) or hypoxia (1% O2) conditions for the indicated times. Cells were subsequently lysed in RIPA lysis buffer containing protease inhibitors and subjected to immunoblotting with primary antibodies

Publication protocol

Cells were cultured in normal culture medium under normoxia (21% O2) or hypoxia (1% O2) conditions for the indicated times. Cells were subsequently lysed in RIPA lysis buffer containing protease inhibitors and subjected to immunoblotting with primary antibodies

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Papers

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Paper title
Selective reversible inhibition of autophagy in hypoxic breast cancer cells promotes pulmonary metastasis
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Manufacturer protocol

Download the product protocol from Novus Biologicals for LC3B Antibody below.

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