Beclin-1 (D40C5) Rabbit mAb

Autophagy assay cell type - C57BL/6

Experiment
Autophagy assay cell type - C57BL/6
Product
Beclin-1 (D40C5) Rabbit mAb from Cell Signaling Technology
Manufacturer
Cell Signaling Technology

Protocol tips

Protocol tips
- Dilute primary Ab at 1:1000

Publication protocol

Western blot analysis was carried out following standard methods. Cells were lysed with RIPA lysis buffer (Cell Signalling Technologies Inc, 9806, USA). Protein detection was performed using Amersham Imager 600 (GE Healthcare Life Sciences) chemiluminescence (Invitrogen, USA). Band intensities were quantified by using the software ImageJ (NIH, USA). LC3 conversion was quantified by measuring band intensities of LC3-II and normalized to β-actin.

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Papers

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Paper title
2-Aminoethoxydiphenylborane sensitizes anti-tumor effect of bortezomib via suppression of calcium-mediated autophagy
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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Beclin-1 (D40C5) Rabbit mAb below.

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