iScript cDNA Synthesis Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Reverse transcribe for 20 min at 46C	For downstream PCR use only 1/10 of the reaction volume (typically 2ul)

Protocol tips
Reverse transcribe for 20 min at 46C
Downstream tips
For downstream PCR use only 1/10 of the reaction volume (typically 2ul)

Publication protocol

Total yeast RNA was extracted as previously described41. 15 μg of purified RNA was treated with the Turbo DNase kit (Ambion) and 1 μg of DNA-free RNA was synthesized into cDNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories). cDNA was diluted 1:10 before the quantitative RT-PCR was performed.

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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for iScript cDNA Synthesis Kit below.

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