FOXA2 Human Gene Knockout Kit

CRISPR Human - Repression FOXA2

Experiment

CRISPR Human - Repression FOXA2

Product

FOXA2 Human Gene Knockout Kit from OriGene

Manufacturer

OriGene

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		it is recommended that you still keep growing or freeze some of the transfected cells without selection; just in case, you need to perform the selection again.

Downstream tips
it is recommended that you still keep growing or freeze some of the transfected cells without selection; just in case, you need to perform the selection again.

Publication protocol

CRISPR/Cas9 system.
The FOXA2 human gene knockout kit via CRISPR was ordered from OriGene (KN204066). Clones were selected using 2 μg/ml puromycin.

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Reviews

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Discussion

4 years ago

Author: Milena Alexeyeva Russian Federation

DNA insert using CRISPR

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Human - Repression FOXA2 using FOXA2 Human Gene Knockout Kit from OriGene.

Paper title

Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer

Full paper

Manufacturer protocol

Download the product protocol from OriGene for FOXA2 Human Gene Knockout Kit below.

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Videos

Check out videos that might be relevant for performing CRISPR Human - Repression FOXA2 using FOXA2 Human Gene Knockout Kit from OriGene. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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