SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes)

Microarray Mice - Cochlea Expression array (labelled)

Experiment
Microarray Mice - Cochlea Expression array (labelled)
Product
SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Protocol tips
To analyze gene expression of each cochlea turn, 12 SurePrint G3 Mouse Exon Microarrays (Agilent Technologies), which were spotted with 165,984 exon probes (24,547 genes), were hybridized to labeled cRNA (4 microarrays were used for each turn sample). Prior to the hybridization step, Cyanine 3-labeled cRNAs were fragmented using 25X fragmentation buffer at 60°C in a water bath for 30 min and then hybridized to a microarray slide for 17 hours at 65°C in a hybridization oven and washed using Gene Expression Wash Buffer (Agilent Technologies).

Publication protocol

To analyze gene expression of each cochlea turn, 12 SurePrint G3 Mouse Exon Microarrays (Agilent Technologies), which were spotted with 165,984 exon probes (24,547 genes), were hybridized to labeled cRNA (4 microarrays were used for each turn sample). Prior to the hybridization step, Cyanine 3-labeled cRNAs were fragmented using 25X fragmentation buffer at 60°C in a water bath for 30 min and then hybridized to a microarray slide for 17 hours at 65°C in a hybridization oven and washed using Gene Expression Wash Buffer (Agilent Technologies).

Full paper   Login or join for free to view the full paper.

Reviews

SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) from Agilent Technologies has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Microarray Mice - Cochlea Expression array (labelled) using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) from Agilent Technologies.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Agilent Technologies for SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Microarray Mice - Cochlea Expression array (labelled) using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms