GeneChip™ Human Genome U133A 2.0 Array

Microarray Comperative genomic hybridization - Human Tumor

Experiment
Microarray Comperative genomic hybridization - Human Tumor
Product
GeneChip™ Human Genome U133A 2.0 Array from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Avoid touching the surface of
the glass with your fingers. Skin oils and other substances, such as
lotions or ink, can fluoresce. If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Oligonucleotide cDNA microarray analysis
RNA purification and hybridization for the microarray analyses were performed as previously described (Jiang, et al 2009). In brief, total RNA from patient tumour specimens was isolated by Trizol reagents (Invitrogen, Carlsbad, CA) and followed by affinity column purification (Qiagen, Valencia, CA). cDNA was generated from 100 ng total RNA using a poly dT oligonucleotide (Affymetrix, Santa Clara, CA). Biotinylated labelling of cRNA was performed by in vitro transcription according to manufacturer’s protocol. Fragmented cRNA (15 µg) was hybridized to HG_U133 A2 Gene Chip® arrays (Affymetrix) using Fluidics Station 450 (Affymetrix).

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Papers

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Paper title
Comparative genomic expression signatures of signal transduction pathways and targets in paediatric Burkitt lymphoma (PEBL): A Children’s Oncology Group (COG) report
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for GeneChip™ Human Genome U133A 2.0 Array below.

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