SurePrint G3 Mouse GE 8x60K Microarray Kit

Microarray Comperative genomic hybridization - Mouse iPSC

Experiment
Microarray Comperative genomic hybridization - Mouse iPSC
Product
SurePrint G3 Mouse GE 8x60K Microarray Kit from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Protocol tips
Each labeled miPSC DNA was hybridized together with the labeled donor DNA onto Agilent SurePrint G3 mouse 1 × 1 M microarray for 40 hours at 65°C. DNA processing, microarray handling and scanning were conducted following the Agilent oligonucleotide CGH protocol (version 6.0).

Publication protocol

Genomic DNAs extracted from each miPSC line and the parental donor (MEF B2) were fragmented using AluI and RsaI enzyme digestion. DNA labeling was conducted using Agilent SureTag DNA Labeling Kit. Different fluorescence dyes were used for DNA labeling of miPSCs (Cy5-dUTP) and the donor parental cell line (Cy3-dUTP). Each labeled miPSC DNA was hybridized together with the labeled donor DNA onto Agilent SurePrint G3 mouse 1 × 1 M microarray for 40 hours at 65°C. DNA processing, microarray handling and scanning were conducted following the Agilent oligonucleotide CGH protocol (version 6.0).

Full paper   Login or join for free to view the full paper.

Reviews

SurePrint G3 Mouse GE 8x60K Microarray Kit from Agilent Technologies has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Microarray Comperative genomic hybridization - Mouse iPSC using SurePrint G3 Mouse GE 8x60K Microarray Kit from Agilent Technologies.

Paper title
Genome-wide CNV analysis in mouse induced pluripotent stem cells reveals dosage effect of pluripotent factors on genome integrity
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Agilent Technologies for SurePrint G3 Mouse GE 8x60K Microarray Kit below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing Microarray Comperative genomic hybridization - Mouse iPSC using SurePrint G3 Mouse GE 8x60K Microarray Kit from Agilent Technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms