Human Genome CGH Microarray Kit 244A

Microarray Comperative genomic hybridization - Human SH-SY5Y

Experiment
Microarray Comperative genomic hybridization - Human SH-SY5Y
Product
Human Genome CGH Microarray Kit 244A from Agilent Technologies
Manufacturer
Agilent Technologies

Protocol tips

Protocol tips
Avoid touching the surface of
the glass with your finersǯ Skin oils and other substances, such as
lotions or ink, can fluoresceǯ If the surface of the glass is noticeably dirty, it can be carefully cleaned with a nonabrasive laboratory tissue.

Publication protocol

Array CGH analysis
Array CGH analysis was conducted using the Human Genome CGH 244K Oligo Microarray Kit (G4411B, Agilent Technologies, Santa Clara, CA, USA). Genomic DNA prepared from primary neuroblastoma tissues or cell lines was labeled with Cy3-dye using a QuickAmp labeling kit. Human placental DNA was labeled with Cy5-dye and used as a reference control. Labeling, hybridization and subsequent data processing by FeatureExtraction and CGH-Analytics software were performed according to the manufacturer's instructions. Relative copy number of the probes surrounding the and genomic locus (from to ) were compared in each primary tumor or cell line.

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Papers

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Paper title
, a -Antisense Gene of , Encodes a Evolved Protein That Inhibits GSK3β Resulting in the Stabilization of MYCN in Human Neuroblastomas
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Manufacturer protocol

Download the product protocol from Agilent Technologies for Human Genome CGH Microarray Kit 244A below.

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