MethylFlash™ Methylated DNA Quantification Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Upstream tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to prevent solutions from sticking to the surface

Publication protocol

DNA Methylation Analysis.
DNA was isolated from SH-SY5Y cell culture after various pretreatments, and any contaminating RNA was removed by RNase treatment. Assessment of global DNA methylation status was accomplished as described previously (Hodgson et al., 2013) using the MethylFlash Methylated DNA Quantification Kit from Epigentek (see the manufacturer’s website for full protocol). The methylated fraction of DNA was identified using 5-methylcytosine monoclonal antibodies and quantified by an enzyme-linked immunosorbent assay–like reaction. The levels of methylated DNA were calculated using the optical density intensity on a microplate reader at 450 nm.

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Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash™ Methylated DNA Quantification Kit below.

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