Quant-iT™ PicoGreen® dsDNA Assay Kit

DNA quantification Human - Hep G2

Experiment
DNA quantification Human - Hep G2
Product
Quant-iT™ PicoGreen® dsDNA Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
To minimize photo bleaching
effects, keep the time for fluorescence measurement constant for all samples.

During incubation at room temperature, protected from light.

Publication protocol

Assessment of hepatocytes viability, proliferation and functionality
AlamarBlue® and PicoGreen® assays
HepG2 metabolic activities, proliferation and growth and scaffold cytotoxicity were assessed using alamarBlue® assay (Molecular Probes®, Invitrogen®) on days 1, 3, 7 and 14. A standard curve was worked out experimentally which suggested 4 h incubation period for the HepG2 cell line to be the optimum timing to measure the FI for alamarBlue®. Further quantification of the cell-line proliferation was made by quantitating double-stranded DNA (dsDNA) using PicoGreen® assay (Quant-iT™ PicoGreen® dsDNA Assay Kit, Molecular Probes®, Invitrogen®) on culture D10 of experiments on PMS and on culture D7 and D14 of experiments on the 3D scaffolds.

FI was measured using a fluorescence plate reader (Fluoroskan Ascent® FL, and Ascent Software, Thermo Labsystems, Helsinki, Finland) at 530 nm excitation wavelength and 620 nm emission wavelength for alamarBlue® and at 485 nm excitation and 538 nm emission for PicoGreen®.

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Discussion

Discussion

2 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - Hep G2 using Quant-iT™ PicoGreen® dsDNA Assay Kit from Thermo Fisher Scientific.

Paper title
Modifying three-dimensional scaffolds from novel nanocomposite materials using dissolvable porogen particles for use in liver tissue engineering
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Quant-iT™ PicoGreen® dsDNA Assay Kit below.

Download PDF Download manufacturer protocol

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