MethylFlash Methylated DNA 5-mC Quantification Kit

DNA quantification Human - WI-38

Experiment
DNA quantification Human - WI-38
Product
MethylFlash Methylated DNA 5-mC Quantification Kit from Epigentek
Manufacturer
Epigentek

Protocol tips

Protocol tips
Do not allow pipette tip to touch the outer edges or inner sides of the wells to
prevent solutions from sticking to the surface

Publication protocol

2.11. DNA and RNA methylation
To measure the levels of 5-methyldeoxycytosine (5-mdC) in DNA, 5-methylcytosine (5-mC) in RNA and N6-methyladenosine (m6A) in RNA, ELISA-based assays were used, namely, MethylFlash Methylated DNA 5-mdC Quantification Kit, MethylFlash 5-mC RNA Methylation ELISA Easy Kit and EpiQuik m6A RNA Methylation Quantification Kit, respectively (EpiGentek, Farmingdale, New York, USA).

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Discussion

Discussion

1 year ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

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Manufacturer protocol

Download the product protocol from Epigentek for MethylFlash Methylated DNA 5-mC Quantification Kit below.

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