Qubit dsDNA HS Assay Kit

DNA quantification Human - BJ

Experiment
DNA quantification Human - BJ
Product
Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Do not label the side of the tube as this could interfere with the sample read.

Publication protocol

Massive parallel sequencing
qPCR-qualified ChIP assays were quantified (Qubit dsDNA HS assay kit, Invitrogen); 3–10 ng of the ChIP material was used for preparing Illumina sequencing libraries following a multiplexing approach (NEXTflexTM ChIP-seq Bioo Scientific, reference 5143-02). Prepared sequencing libraries were sequenced on an Illumina HiSeq2000 instrument. Regular Illumina pipelines were used for image processing and base calling. Sequence files were then aligned to the human genome assembly using default parameters (hg19; Bowtie).

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Reviews

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Discussion

Discussion

3 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - BJ using Qubit dsDNA HS Assay Kit from Thermo Fisher Scientific.

Paper title
Reconstruction of gene regulatory networks reveals chromatin remodelers and key transcription factors in tumorigenesis
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Qubit dsDNA HS Assay Kit below.

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Videos

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