7-AAD (7-Aminoactinomycin D)

DNA quantification Human - PC-3

Experiment
DNA quantification Human - PC-3
Product
7-AAD (7-Aminoactinomycin D) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
incubate cells on ice only

Publication protocol

Annexin V-PE/7AAD staining
PC cells were grown at a density of 50% confluence in 100-mm culture dishes and were treated with varying concentrations of the drugs for 48 hours. Cells were stained using Annexin V-PE apoptosis detection kit (BD-Pharmingen) according to the manufacturer’s recommendations. Briefly, the cells were washed in PBS and resuspended in binding buffer (1×106 per ml). 5µl of AnnexinV-PE and 5µl of 7-AAD (7-aminoactinomycin-D) were added and incubated for 15 minutes at room temperature in the dark and analyzed by flow cytometry.



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Discussion

Discussion

2 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - PC-3 using 7-AAD (7-Aminoactinomycin D) from Thermo Fisher Scientific.

Paper title
New potential anti-cancer agents synergize with bortezomib and ABT-737 against prostate cancer
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for 7-AAD (7-Aminoactinomycin D) below.

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