Wizard® Genomic DNA Purification Kit

DNA isolation / purification Cells - primary cells mouse embryonic fibroblast (MEF)

Experiment
DNA isolation / purification Cells - primary cells mouse embryonic fibroblast (MEF)
Product
Wizard® Genomic DNA Purification Kit from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
For blood DNA isolation, blood must be collected in EDTA, heparin or citrate anticoagulant tubes to prevent clotting.

Publication protocol

Bisulfite DNA sequencing
Genomic DNA used for bisulfite conversion was extracted using the Wizard Genomic DNA Purification Kit (Promega, PRA1125) according to the manufacturer’s protocol. All DNA sequences for primer design were obtained from the UCSC genome browser (http://genome.ucsc.edu). Primer design and CpG island (CGI) prediction was performed using the MethPrimer software29 (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). This software scores a DNA sequence as a CGI when it fulfills the following criteria: at least a 200-bp sequence, with a G+C content >50%, and an Obs/Exp CpG ratio >0.6. Bisulfite conversion was done using the EZ DNA Methylation Gold Kit (Zymo Research, D5005). Up to 2 μg of genomic DNA was converted using the C1000 Thermal Cycler (Bio-Rad) at 98 °C for 10 min, followed by 64 °C for 2.5 h.

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Discussion

Discussion

1 year ago

Author: R. Verma India

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Cells - primary cells mouse embryonic fibroblast (MEF) using Wizard® Genomic DNA Purification Kit from Promega.

Paper title
E2f6-mediated repression of the meiotic and genes during early embryonic development requires Ezh2 and not the de novo methyltransferase Dnmt3b
Full paper
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Manufacturer protocol

Download the product protocol from Promega for Wizard® Genomic DNA Purification Kit below.

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