ROS-Glo™ H2O2 Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were pretreated with lipid and/or doxorubicin	- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation

Upstream tips
- Cells were pretreated with lipid and/or doxorubicin
Protocol tips
- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation

Publication protocol

Intracellular ROS levels were assessed using the ROS-Glo™ H2O2 assay from Promega (Southampton, UK) following the manufacturer's instructions. Briefly, Huh7 cells were exposed to lipid and/or doxorubicin as described previously; during the final 6 hours of incubation, 25 μM H2O2 substrate solution was added to the cells. At the end of incubation time, ROS-Glo Detection solution was added, incubated for 20 minutes and then luminescence was measured using a BMG LABTECH FLUOstar Omega plate reader (Ortenberg, Germany).

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Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

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