ROS-Glo™ H2O2 Assay

ROS assay cell type - Huh7

Experiment
ROS assay cell type - Huh7
Product
ROS-Glo™ H2O2 Assay from Promega
Manufacturer
Promega

Protocol tips

Upstream tips
- Cells were pretreated with lipid and/or doxorubicin
Protocol tips
- 25 μM H2O2 substrate solution was added to the cells during the last 6h of incubation

Publication protocol

Intracellular ROS levels were assessed using the ROS-Glo™ H2O2 assay from Promega (Southampton, UK) following the manufacturer's instructions. Briefly, Huh7 cells were exposed to lipid and/or doxorubicin as described previously; during the final 6 hours of incubation, 25 μM H2O2 substrate solution was added to the cells. At the end of incubation time, ROS-Glo Detection solution was added, incubated for 20 minutes and then luminescence was measured using a BMG LABTECH FLUOstar Omega plate reader (Ortenberg, Germany).

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Papers

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Paper title
Synergistic interaction between lipid-loading and doxorubicin exposure in Huh7 hepatoma cells results in enhanced cytotoxicity and cellular oxidative stress: implications for acute and chronic care of obese cancer patients
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Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

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