ROS-ID® Total ROS/Superoxide detection kit

Protocol tips

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	- After 24h of cell seeding, cells were treated with ROS detection solution and incubate under normal culture conditions for 1 h. - After washing cells off from reagent, cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidant

Protocol tips
- After 24h of cell seeding, cells were treated with ROS detection solution and incubate under normal culture conditions for 1 h. - After washing cells off from reagent, cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidant

Publication protocol

The level of intracellular ROS generation was assessed with the total ROS detection kit (Enzo Life) by following the manufacturer's instructions. Cells were seeded in a 24-well culture plate. After 24 h, cells were loaded with the ROS detection solution and incubate under normal culture conditions for 1 h. After carefully removing the ROS detection solution and cells were treated with the solvent or Alternol in the presence or absence of the anti-oxidants as indicated in the figures. There are three replicated wells for each group. After careful wash with the washing buffer cells were immediately observed and microscopic images were taken under a fluorescence microscope (Olympus, Japan).

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS/Superoxide detection kit below.

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