Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)

Protocol tips

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	- Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C.

Protocol tips
- Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C.

Publication protocol

Nitric oxide production was assessed by measuring the levels of oxidized forms (nitrites and nitrates) in samples, using a nitric oxide assay kit (Abcam, Cambridge, UK). Mitochondrial ROS levels were analyzed using the Elite Mitochondrial ROS Activity Assay Kit (eEnzyme, Gaithersburg, MD). Cells were harvested and incubated with 100 μL Elite ROS Deep Red stain solution on 96-well plates for 60 min at 37°C. Fluorescence intensity was measured at EX/EM = 650/675 nm.

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