CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - human MCF-7

Experiment
Wound healing assay cell type - human MCF-7
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Downstream tips
- Cells are visualised on days 0, 1, 2 and 3 to monitor migration of the cells into the wound field.

Publication protocol

Claudin-3 siRNA-transfected and mock transfected control cells were plated at 2.5×105 cells per well into Cytoselect wound-healing assay plates (catalog no. CBA-120; Cell Biolabs San Diego, CA, USA) and grown until 95% confluent. Each well contained an insert that created a defined, rectangular wound field. Following removal of the inserts, the cells were stained (stain included in Cytoselect kit) and photographed on days 0, 1, 2 and 3 to monitor migration of the cells into the wound field. Protein extracts were prepared from the mock- and siRNA-transfected cells (as described previously) on days 0 and 3, and subjected to western blot analysis to assess the degree of claudin-3 suppression.

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Reviews

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Discussion

Discussion

2 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MCF-7 using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Paper title
Overexpression and delocalization of claudin-3 protein in MCF-7 and MDA-MB-415 breast cancer cell lines
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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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