Oris™ Universal Cell Migration Assembly Kit, 96 wells

Wound healing assay cell type - human MCF-7

Experiment
Wound healing assay cell type - human MCF-7
Product
Oris™ Universal Cell Migration Assembly Kit, 96 wells from Platypus Technologies
Manufacturer
Platypus Technologies

Protocol tips

Protocol tips
- At step-14, incubate cells for 16–18 h and at Step 15 stain with 0.5% crystal violet

Publication protocol

Cell migration assay was conducted by Oris Universal Cell Migration Assembly kit (Platypus Technologies, LCC, Madison, WI, USA) following manufacture’s protocol. Briefly, single cell suspension (5 × 104 cells/well/100 μL) was loaded into stopper loaded wells and incubated overnight to permit cell attachment. To start cell migration, the stoppers was removed, wash wells gently with PBS, added complete cell culture medium and incubate for 16–18 h. Pictures of wells were captured with inverted microscopy after fixation and stain with 0.5% crystal violet (Sigma-Aldrich)/50% EtOH. Data were analyzed with ImageJ software (National Institute of Health, Bethesda, MA, USA).


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Reviews

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Discussion

Discussion

2 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MCF-7 using Oris™ Universal Cell Migration Assembly Kit, 96 wells from Platypus Technologies.

Paper title
Methyl Antcinate A suppresses the population of cancer stem-like cells in MCF7 human breast cancer cell line.
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Manufacturer protocol

Download the product protocol from Platypus Technologies for Oris™ Universal Cell Migration Assembly Kit, 96 wells below.

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