CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - human gHMVEC (glioma human microvascular endothelial cells)

Experiment
Wound healing assay cell type - human gHMVEC (glioma human microvascular endothelial cells)
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- Cells were washed with serum-free media, and cells were treated or not with NGF (200 ng/mL) containing or not inhibitors of α9β1 integrin.
Downstream tips
- Cells are pictured at 0 and 24 h

Publication protocol

Wound healing assay was performed using CytoSelect kit (Cell Biolabs). Cells were seeded on 24-well plate containing inserts for artificial wound creation (0.9 mm). The next day, inserts were removed, wells were washed with serum-free media, and cells were treated or not with NGF (200 ng/mL) containing or not inhibitors of α9β1 integrin. For positive control, cells were treated with 2% FBS. Progression of wound closing was monitored by taking phase-contrast pictures at a magnification of 100× at times 0 and 24 h. Images were analyzed using ImageJ software. The percentage of wound closure was calculated after 24 h and normalized to the wound area at time 0

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Discussion

Discussion

2 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human gHMVEC (glioma human microvascular endothelial cells) using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Paper title
Importance of interaction between nerve growth factor and α9β1 integrin in glial tumor angiogenesis
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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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