CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - human RT-7

Experiment
Wound healing assay cell type - human RT-7
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- Cells were treated with various concentrations (0, 0.1, 1, 10 ng/ml) of leptin before creating wound.
Downstream tips
- Images are captured at 0, 12, 24, and 36 hours after the removal of the inserts

Publication protocol

The effect of leptin on the migration of RT7 cells was analyzed with a CytoSelect Wound Healing Assay kit (Cell Biolabs Inc., San Diego, USA). The assay was performed according to the manufacturer’s instructions. In brief, RT7 cells were seeded into 24-well plates and cultured overnight. After the cells were treated with various concentrations (0, 0.1, 1, 10 ng/ml) of leptin, the inserts, which were equipped to make a 0.9 mm gap to simulate a wound, were removed from the wells to begin the wound healing assay. Each gap was measured at 0, 12, 24, and 36 hours after the removal of the inserts.

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Reviews

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Discussion

Discussion

1 year ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human RT-7 using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

Paper title
Leptin Promotes Wound Healing in the Oral Mucosa
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Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

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