IncuCyte® Cell Migration Kit

Wound healing assay cell type - human A375

Experiment
Wound healing assay cell type - human A375
Product
IncuCyte® Cell Migration Kit from Essen Bioscience
Manufacturer
Essen Bioscience

Protocol tips

Downstream tips
- Cell migration was monitored every 2 h for 2 days.

Publication protocol

Migration of model cell lines overexpressing CD271 (A375NGFR/CD271; MeWoPar-NGFR/CD271) or controls (A375GFP; MeWoPar-GFP), as well as fotemustine or vindesine resistant cells (MeWoFote; MeWoVind) was performed with the scratch wound cell migration assay (Essen Bioscience Ltd, Welwyn Garden City, Hertfordshire, UK) following the manufacturer's instructions. Briefly, 30 000 cells of each cell line were seeded 24 h before, yielding in a dense cell layer. Reproducibly wounds were scratched with the Wound Maker, a block with 96 pins. After wounding, the 96-well plate was placed into the Incucyte ZOOM life-cell imaging system (Essen Bioscience Ltd.). Cell migration was monitored every 2 h for 2 days, using a × 10 magnification. Serial pictures were stacked for movie preparation using the ImageJ software (https://imagej.nih.gov/ij/). Statistical analysis was performed by using a two-tailed, paired t-test.

Full paper   Login or join for free to view the full paper.

Reviews

IncuCyte® Cell Migration Kit from Essen Bioscience has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

1 year ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human A375 using IncuCyte® Cell Migration Kit from Essen Bioscience.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Essen Bioscience for IncuCyte® Cell Migration Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Wound healing assay cell type - human A375 using IncuCyte® Cell Migration Kit from Essen Bioscience. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms