IncuCyte® Cell Migration Kit

Wound healing assay cell type - human MEWO

Experiment
Wound healing assay cell type - human MEWO
Product
IncuCyte® Cell Migration Kit from Essen Bioscience
Manufacturer
Essen Bioscience

Protocol tips

Downstream tips
- Cell migration was monitored every 2 h for 2 days.

Publication protocol

Migration of model cell lines overexpressing CD271 (A375NGFR/CD271; MeWoPar-NGFR/CD271) or controls (A375GFP; MeWoPar-GFP), as well as fotemustine or vindesine resistant cells (MeWoFote; MeWoVind) was performed with the scratch wound cell migration assay (Essen Bioscience Ltd, Welwyn Garden City, Hertfordshire, UK) following the manufacturer's instructions. Briefly, 30 000 cells of each cell line were seeded 24 h before, yielding in a dense cell layer. Reproducibly wounds were scratched with the Wound Maker, a block with 96 pins. After wounding, the 96-well plate was placed into the Incucyte ZOOM life-cell imaging system (Essen Bioscience Ltd.). Cell migration was monitored every 2 h for 2 days, using a × 10 magnification. Serial pictures were stacked for movie preparation using the ImageJ software (https://imagej.nih.gov/ij/). Statistical analysis was performed by using a two-tailed, paired t-test.

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Discussion

Discussion

4 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - human MEWO using IncuCyte® Cell Migration Kit from Essen Bioscience.

Paper title
The role of the cancer stem cell marker CD271 in DNA damage response and drug resistance of melanoma cells
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Manufacturer protocol

Download the product protocol from Essen Bioscience for IncuCyte® Cell Migration Kit below.

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