CytoSelect™ 24-Well Wound Healing Assay

Wound healing assay cell type - rat MSC

Experiment
Wound healing assay cell type - rat MSC
Product
CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs
Manufacturer
Cell Biolabs

Protocol tips

Protocol tips
- After cells are washed, weels are filled with with MSC-CM, MIV, MSC-CM-dep MIV, 30% FBS, or serum-free DMEM (DMEM []) and incubated at 37°C for 48 hours.

Publication protocol

The migratory properties of Zol rMSCs were examined using the CytoSelect Wound Healing Assay kit (Cell Biolabs, San Diego, CA. USA) according to the manufacturer’s instructions. In brief, cell suspension was added to the well using a plastic insert. The insert was removed from the well after a monolayer of cells had formed, creating a wound gap of 0.9 mm. After washing, the cells were incubated at 37°C for 48 hours with MSC-CM, MIV, MSC-CM-dep MIV, 30% FBS, or serum-free DMEM (DMEM []). The extent of wound closure was determined using a light microscope (CK40; Olympus, Tokyo, Japan).

Full paper   Login or join for free to view the full paper.

Reviews

CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

3 years ago

Author: United States

Which cell lines would you recommend for wound healing assays?

I will be studying some properties of cellulose acetate-PLA blend nanofibers. Which cell lines would you recommend for running wound healing assays effectively?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Wound healing assay cell type - rat MSC using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Cell Biolabs for CytoSelect™ 24-Well Wound Healing Assay below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Wound healing assay cell type - rat MSC using CytoSelect™ 24-Well Wound Healing Assay from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms