|siRNA concentration-10, 50 and 100 nM.
sense strand 5′-GCUCAGCAGGAAAGGUGUUUU-3′ and antisense strand 5′-AACACCUUUCCUGCUGAGCUU-3′
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).
Incubate for 5 minutes at room temperature and add to cells.
Incubate cells for 1–3 days at 37°C.
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