Dusp3 siRNA

siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3

Experiment
siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3
Product
Dusp3 siRNA from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
siRNA concentration-50 nM

Incubate cells for another 24h at 37°C.

Publication protocol

To test the role of each candidate gene on cytokine production by host defense cells, we transfected siRNAs into the mouse macrophages. All siRNAs were purchased from Invitrogen. RAW264.7 cells or bone-marrow derived macrophages from C57BL/6J were transfected with 50 nM siRNA by Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Twenty-four hours post-transfection, cells were treated with S. aureus Bioparticles (Invitrogen) to a final concentration of 10 µg/ml. At 24 hours post-infection, supernatants were collected and stored at −80°C for Luminex-multiplex cytokine assay. In parallel experiments, cells at 3 hours post-infection were harvested for RNA extraction by RNeasy (QIAGEN), RT-PCR by SuperScript II (Invitrogen) and SYBR Green qPCR analysis (ABI) respectively. Experiments were repeated at least three times. A full list of gene names and siRNA ID numbers were in Table S1.

Full paper   Login or join for free to view the full paper.

Reviews

Dusp3 siRNA from Thermo Fisher Scientific has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3 using Dusp3 siRNA from Thermo Fisher Scientific.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Dusp3 siRNA below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3 using Dusp3 siRNA from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms