Kdm2b siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for 1–3 days at 37°C.

Protocol tips
Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for 1–3 days at 37°C.

Publication protocol

The duplexes of each small interfering RNA (siRNA), targeting murine Fbxl10 mRNA (MSS219951 and MSS219952; Invitrogen), Skp1 (sc-36498A; Santa Cruz Biotechnology), Cul1 (MSS219122 and MSS219123; Invitrogen), or Ring1B (sc-62947A and sc-62947C; Santa Cruz Biotechnology) and control siRNA (Santa Cruz Biotechnology sc-44230 or Invitrogen Stealth RNAi, Negative Control Low GC Duplexes 12935-200) were transfected into cells using Lipofectamine RNAi MAX reagent (Invitrogen) as described (8, 9).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - 3T3-L1 Fbxl10 using Kdm2b siRNA from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Kdm2b siRNA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - 3T3-L1 Fbxl10 using Kdm2b siRNA from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.