Atf4 siRNA(m)

siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4

Experiment
siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4
Product
Atf4 siRNA(m) from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
siRNA conentration-50 nm

Add X-tremeGENE HP Reagent : DNA complexes to cells and incubate for 48h.

Post 48h, induce cells for differentiation and again transfect to achieve persistent inhibition.

Publication protocol

Predesigned mouse Stealth Select RNAi™ siRNA (catalog number MSS202234; Invitrogen) was used to specifically knockdown ATF4 in 3T3‐L1 cells. The Stealth RNAi™ siRNA Negative Control Med GC (catalog number 12935300; Invitrogen) was carried out as a control. 3T3‐L1 cells were transfected with each siRNA (50 nm) using X‐tremeGENE HP transfection reagent (Roche, Basel, Switzerland) when reaching approximately 90% confluence. Two days after transfection, cells were induced to differentiate as described above. Two days after the induction of differentiation, another transfection was performed to achieve persistent inhibition of ATF4 protein synthesis as described by Cignarelli et al. [42].

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Papers

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Paper title
Activating transcription factor 4 regulates adipocyte differentiation via altering the coordinate expression of CCATT/enhancer binding protein β and peroxisome proliferator-activated receptor γ.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Atf4 siRNA(m) below.

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