Jun siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	c-Jun siRNA-5 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for another 24h at 37°C and quantify.

Protocol tips
c-Jun siRNA-5 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells. Incubate cells for another 24h at 37°C and quantify.

Publication protocol

Neuro2a cells were transfected with 5 nM c-Jun siRNA (siRNA ID: s201552; Ambion, Austin, TX, USA), 5 nM WDR35 siRNA (siRNA ID: s93029; Ambion), or 10 nM control siRNA (negative control #1 siRNA, catalog no. 4390843; Ambion) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions. Effects of siRNA on the expression of c-Jun or WDR35 mRNA were tested with qPCR after 24 h of transfection.

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Manufacturer protocol

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