iBONi siRNA pool

siRNA / miRNA gene silencing Mouse - B16-F10 P53

Experiment
siRNA / miRNA gene silencing Mouse - B16-F10 P53
Product
iBONi siRNA pool from Riboxx
Manufacturer
Riboxx

Protocol tips

Protocol tips
siRNA concentration of 50 nM

Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells.

After 48h of transfection treat cells with climacostol (30 μg/ml) for 24h.

Publication protocol

According to the manufacturer’s protocol, iBONI siRNA Pool (a mix of 3 RNA duplex - small interfering RNAs) (Riboxx, Radebeul, Germany) targeting mouse p53 (gene ID 22059; Supplementary Table S2) was mixed to Lipofectamine RNAiMax transfection reagent (Life Technologies, Monza, Italy). iBONI siRNA Pool negative control (non-targeting siRNA; Supplementary Table S2) (Riboxx, Radebeul, Germany) was also used. The mix was added to cultured B16-F10 cells at a siRNA concentration of 50 nM. At 48 h after transfection, climacostol (24 h, 30 μg/ml) was then added before Western blotting and flow cytometry analyses.

Full paper   Login or join for free to view the full paper.

Reviews

iBONi siRNA pool from Riboxx has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Mouse - B16-F10 P53 using iBONi siRNA pool from Riboxx.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Riboxx for iBONi siRNA pool below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Mouse - B16-F10 P53 using iBONi siRNA pool from Riboxx. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms