Vps13a siRNA

siRNA / miRNA gene silencing Rat - PC12 chorein

Experiment
siRNA / miRNA gene silencing Rat - PC12 chorein
Product
Vps13a siRNA from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Seed 2 x 10^5 cells and culture for 24 h at 37°C, 5% CO2.
Protocol tips
Mix diluted RNA and diluted siPORT Amine agent and
incubate for 10 min at room temp.

Transfect cells with mixed transfection complexes and incubate for 72 h.

Publication protocol

PC12 cells were seeded in 6 well plates on a density of 2 x 105 and cultured under standard culture conditions (37°C, 5% CO2) for 24 h. The cells were subsequently transfected for 72 h with validated siRNA for VPS13A (chorein) (ID#s235043, Ambion, Darmstadt, Germany) or with a negative control siRNA (ID#s4390843, Ambion) using siPORT amine transfection agent (Ambion) according to the manufacturer's protocol. The efficiency of silencing was checked by RT-PCR

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Rat - PC12 chorein using Vps13a siRNA from Thermo Fisher Scientific.

Paper title
Chorein Sensitive Dopamine Release from Pheochromocytoma (PC12) Cells.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Vps13a siRNA below.

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