Rn_Sod2_4 FlexiTube siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	siRNA sequence: Sense 5′-GGCUUACUAUUAAACAUUATT-3′ and Antisense 5′-UAAUGUUUAAUAGUAAGCCTA-3′. Incubate cells for 24 h at 37°C with Lipofectamine complexes. After 24 h at 37°C incubate for another 24 h at 37°C with drugs.

Protocol tips
siRNA sequence: Sense 5′-GGCUUACUAUUAAACAUUATT-3′ and Antisense 5′-UAAUGUUUAAUAGUAAGCCTA-3′. Incubate cells for 24 h at 37°C with Lipofectamine complexes. After 24 h at 37°C incubate for another 24 h at 37°C with drugs.

Publication protocol

SOD2-siRNA and scrambled- (SC-) siRNA were purchased from QIAGEN (Germany). The sentence and anti-sentence were as follows: 5′-GGCUUACUAUUAAACAUUATT-3′ and 5′-UAAUGUUUAAUAGUAAGCCTA-3′. Briefly, SOD2 and SC-siRNA oligomers were diluted in DMEM medium without FBS and antibiotics and incubated for 5 min at room temperature. The SC-siRNA served as the negative control. The oligomers were then combined with diluted Lipofectamine 2000, mixed gently, and incubated for 20 min to allow complex formation. During this incubation, the cell culture medium was removed from the plate and the cells were washed twice with phosphate buffered saline (PBS) at 37°C to remove the traces of FBS. The siRNA-Lipofectamine complexes were added to the plate. The cells were incubated for 24 h at 37°C. Then, the cells were washed with PBS and incubated in the medium containing different drugs for 24 h at 37°C.

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Manufacturer protocol

Download the product protocol from Qiagen for Rn_Sod2_4 FlexiTube siRNA below.

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