Fabp5 siRNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Use siRNA at 100 μM in water. Change medium to 100 μM in water after 24h of transfection and incubate for another 3–5 days.

Protocol tips
Use siRNA at 100 μM in water. Change medium to 100 μM in water after 24h of transfection and incubate for another 3–5 days.

Publication protocol

To knock down E-FABP in NGFDPC12 cells, we selected Ambion in vivo siRNA system (Ambion, Austin, TX, USA). Rat FABP5 siRNA (s139047) and negative control siRNA with scrambled sequences were acquired (Life Technologies) and introduced into cells by X-tremeGENE siRNA transfection reagent (Roche Applied Science). The siRNA transfection, following the manufacturer's instruction, was performed as below: siE-FABP or siControl stock was prepared at 100 μM in water and 2 μL of stock was diluted with 250 μL of Opti-MEM. Transfection reagent (10 μL) was also diluted with 250 μL of Opti-MEM. Diluted siRNA and transfection reagent were combined and incubated for 20 min. Three-day-differentiated PC12 cells in 6-well plates were change to antibiotics-free medium (1.5 mL/well) and then transfection solution (0.5 mL) was added to the well. After 24 h, the medium was changed to 1% FBS–NGF medium for continual differentiation for another 3–5 days. The transfected NGFDPC12 cells were treated with PAM accordingly.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Fabp5 siRNA below.

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