TruSeq RNA Library Prep Kit v2

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

RNA was harvested from treated SH-SY5Y cells using the NucleoSpin RNA/Protein kit (Macherey-Nagel GmbH). Library construction and RNA sequencing were performed by the Duke-NUS Genome Biology Facility (DGBF). Briefly, 2.2 μg of RNA was sent for library construction. Quality of RNA was analyzed with the Agilent Bioanalyzer and RNA with RIN > 9 was used. Following poly-A enrichment, recovered RNA was processed using the Illumina TruSeq RNA Sample Preparation Kit v2 protocol (non-stranded) to generate adaptor-ligated libraries. A total of six samples were sequenced, obtained from undifferentiated and differentiated SH-SY5Y cells treated with (i) vehicle-treated control, (ii) 3 μg/ml Bacopa for 24 h, and (iii) 10 μg/ml Bacopa for 4 h. Samples were sequenced using two lanes of an Illumina HiSeq2000 sequencer using 76-bp paired-end reads.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Human - SH-SY5Y using TruSeq RNA Library Prep Kit v2 from Illumina.

Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Human - SH-SY5Y using TruSeq RNA Library Prep Kit v2 from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.