TruSeq Stranded mRNA

RNA sequencing Human - PANC-1

Experiment
RNA sequencing Human - PANC-1
Product
TruSeq Stranded mRNA from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
The protocol has been tested using 0.1–1 µg of high-quality universal human reference total RNA as input.

Publication protocol

PANC-1 (0.35 × 106 cells/well) cell line was seeded overnight in 60 mm plates. Next day, cells were treated with 400 nM CM03 for 6 and 24 h. For gemcitabine treatment, cells were treated for 6 and 24 h with DMSO or 30 nM, 100 nM, 300 nM, 1 μM, 3 μM, and 10 μM gemcitabine-HCL for PANC-1 cells and 30 nM, 300 nM, and 3 μM gemcitabine-HCl for MIA PaCa-2 cells. Total RNA was extracted using the Qiagen RNeasy Plus Mini Kit (ThermoFisher, catalogue no. 74134) as per the manufacturer’s instructions. RNA quality (RIN > 9.0) was checked with an Aligent 2100 bioanalyzer RNA 6000 Nano Chip, and RNA concentration was quantified using a Qubit fluorometer (ThermoFisher) and Qubit RNA HS Assay Kit (Qiagen, catalogue no. Q32852). RNA-seq libraries were then generated using the Illumina Truseq RNA HT (stranded mRNA) kit (Illumina, catalogue no. RS-122–2103) as per the manufacturer’s instructions and sequenced using an Illumina HiSeq4000.

Full paper   Login or join for free to view the full paper.

Reviews

TruSeq Stranded mRNA from Illumina has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Human - PANC-1 using TruSeq Stranded mRNA from Illumina.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Illumina for TruSeq Stranded mRNA below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Human - PANC-1 using TruSeq Stranded mRNA from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms