TruSeq RNA Library Prep Kit v2

RNA sequencing Human - MIA PaCa-2

Experiment
RNA sequencing Human - MIA PaCa-2
Product
TruSeq RNA Library Prep Kit v2 from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

RNA-seq was performed for MIA PaCa-2 and PANC-1 cells treated with 5-aza-dC or IR exposure (2 Gy and 4 Gy) and both combination. Briefly, total RNA was extracted from MIA PaCa-2 and PANC-1 cell lines with different conditions. For the Illumina library preparations, the extracted RNAs was synthesized using TruSeq RNA sample preparation kit (Illumina Inc., CA, USA) and sequenced using Illumina HiSeq 2000. Paired-end reads were trimmed by quality score using Trimmomatic and mapped to the human reference genome (hg38) using HISAT2. Gene expression levels were quantified by means of the reads per kilo base per million mapped reads (RPKM) method. Differentially expressed genes between different conditions were identified using the GFOLD algorithm. Lowly expressed genes which were below 1 RPKM were discarded.

Full paper   Login or join for free to view the full paper.

Reviews

TruSeq RNA Library Prep Kit v2 from Illumina has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Human - MIA PaCa-2 using TruSeq RNA Library Prep Kit v2 from Illumina.

Paper title
Combinatorial effects of an epigenetic inhibitor and ionizing radiation contribute to targeted elimination of pancreatic cancer stem cell
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA sequencing Human - MIA PaCa-2 using TruSeq RNA Library Prep Kit v2 from Illumina. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms