TruSeq RNA Library Prep Kit v2

RNA sequencing Human - A413

Experiment
RNA sequencing Human - A413
Product
TruSeq RNA Library Prep Kit v2 from Illumina
Manufacturer
Illumina

Protocol tips

Protocol tips
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Publication protocol

Total RNA from A413 cells was isolated using TRI REAGENT™ (Sigma-Aldrich) according to the manufacturer’s instructions. RNA quantity was measured with a spectrometer (Nanodrop ND 1000), and RNA quality was analysed on the Agilent 2100 Bioanalyzer using the RNA 6000 Nano Chip (Agilent Technologies, USA). We only included RNA samples with an RIN value above 8. Indexed cDNA libraries were generated using TruSeq RNA Sample Preparation kits v2 (Illumina, USA) according to the manufacturer’s protocol. The average library size was 300 bp as determined on the Agilent 2100 Bioanalyzer with DNA 1000 Chips.

The libraries were sequenced on the Illumina HiScanSQ Sequencing System (Interdisciplinary Centre for Clinical Research, Leipzig), generating on average 11.8 ± 1.5 million 101-bp raw paired-end reads per sample on one flow cell lane.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA sequencing Human - A413 using TruSeq RNA Library Prep Kit v2 from Illumina.

Paper title
Prostaglandin E glyceryl ester is an endogenous agonist of the nucleotide receptor P2Y
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Manufacturer protocol

Download the product protocol from Illumina for TruSeq RNA Library Prep Kit v2 below.

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